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CoraLite®594-conjugated ROR1 Monoclonal antibody

ROR1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F5D8

Cat No : CL594-66923

Print datasheet

Synonyms

dJ537F10.1, NTRKR1, ROR1

验证数据展示

  • FC experiment of K-562 using CL594-66923

    FC experiment of K-562 using CL594-66923

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ROR1 (CL594-66923, Clone:1F5D8) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ROR1 Ready-To-Use IHC Kit. ROR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66923-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66923 targets ROR1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen ROR1 fusion protein Ag14586 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor tyrosine kinase-like orphan receptor 1
Synonyms dJ537F10.1, NTRKR1, ROR1
Calculated Molecular Weight 937 aa, 104 kDa
GenBank Accession NumberBC006374
Gene Symbol ROR1
Gene ID (NCBI) 4919
RRIDAB_2920057
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01973
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

实验方案

Product Specific Protocols
FC protocol for CL594 ROR1 antibody CL594-66923Download protocol
Standard Protocols
Click here to view our Standard Protocols