• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ROR1 Monoclonal antibody

ROR1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5D8

Cat No : 66923-1-Ig

Print datasheet

Synonyms

dJ537F10.1, NTRKR1, ROR1

验证数据展示

  • WB analysis using 66923-1-Ig

    WB analysis using 66923-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • FC experiment of K-562 using 66923-1-Ig

    FC experiment of K-562 using 66923-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human ROR1 (66923-1-Ig, Clone:1F5D8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human breast cancer using 66923-1-Ig

    IHC staining of human breast cancer using 66923-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ROR1 antibody included
  • IHC staining of human breast cancer using 66923-1-Ig

    IHC staining of human breast cancer using 66923-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ROR1 antibody included
  • WB analysis of NIH/3T3 using 66923-1-Ig

    WB analysis of NIH/3T3 using 66923-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, K-562 cells, NCI-H1299 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ROR1 Ready-To-Use IHC Kit. ROR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66923-1-Ig targets ROR1 in WB, FC, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen ROR1 fusion protein Ag14586 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor tyrosine kinase-like orphan receptor 1
Synonyms dJ537F10.1, NTRKR1, ROR1
Calculated Molecular Weight 937 aa, 104 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC006374
Gene Symbol ROR1
Gene ID (NCBI) 4919
RRIDAB_2882250
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ01973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

实验方案

Product Specific Protocols
WB protocol for ROR1 antibody 66923-1-IgDownload protocol
IHC protocol for ROR1 antibody 66923-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

SETD8 induces stemness and epithelial-mesenchymal transition of pancreatic cancer cells by regulating ROR1 expression.

Authors - Mengqi Liu
  • KD Validated
View Article
humanIHC

Int Immunopharmacol

ROR1/STAT3 positive feedback loop facilitates cartilage degeneration in Osteoarthritis through activation of NF-κB signaling pathway

Authors - Wei Huang
View Article