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验证数据展示

  • WB analysis using 84772-6-RR

    WB analysis using 84772-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84772-6-RR (RNF126 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84772-6-RR

    IHC staining of human placenta using 84772-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse ovary using 84772-6-RR

    IHC staining of mouse ovary using 84772-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 84772-6-RR

    IHC staining of mouse pancreas using 84772-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 84772-6-RR

    IHC staining of rat pancreas using 84772-6-RR

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 84772-6-RR

    FC experiment of Jurkat using 84772-6-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Recombinant antibody (84772-6-RR, Clone:242215A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84772-6-RR

    Affinity and Kinetic Characterization of 84772-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84772-6-RR against Human RNF126 were performed. The affinity constant is below 1 pM.

RNF126 Recombinant antibody

RNF126 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84772-6-RR

产品说明书

CloneNo. 242215A10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inrat pancreas tissue, human ovary cancer tissue, human placenta tissue, mouse ovary tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84772-6-RR targets RNF126 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF126 fusion protein Ag13816 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 126
别名 242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126
计算分子量 326 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC001442
基因名称 RNF126
Gene ID (NCBI) 55658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

实验方案

Product Specific Protocols
WB protocol for RNF126 antibody 84772-6-RRDownload protocol
IHC protocol for RNF126 antibody 84772-6-RRDownload protocol
FC protocol for RNF126 antibody 84772-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols