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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84772-6-RR (same clone as 84772-6-PBS)

    WB analysis using 84772-6-RR (same clone as 84772-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84772-6-RR (RNF126 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84772-6-RR (same clone as 84772-6-PBS)

    IHC staining of human placenta using 84772-6-RR (same clone as 84772-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 84772-6-RR (same clone as 84772-6-PBS)

    IHC staining of mouse ovary using 84772-6-RR (same clone as 84772-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 84772-6-RR (same clone as 84772-6-PBS)

    IHC staining of mouse pancreas using 84772-6-RR (same clone as 84772-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • IHC staining of rat pancreas using 84772-6-RR (same clone as 84772-6-PBS)

    IHC staining of rat pancreas using 84772-6-RR (same clone as 84772-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84772-6-RR (same clone as 84772-6-PBS)

    FC experiment of Jurkat using 84772-6-RR (same clone as 84772-6-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Recombinant antibody (84772-6-RR, Clone:242215A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84772-6-RR

    Affinity and Kinetic Characterization of 84772-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84772-6-RR against Human RNF126 were performed. The affinity constant is below 1 pM.

RNF126 Recombinant antibody, PBS Only

RNF126 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84772-6-PBS

产品说明书

CloneNo. 242215A10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84772-6-PBS targets RNF126 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF126 fusion protein Ag13816 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 126
别名 242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126
计算分子量 326 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC001442
基因名称 RNF126
Gene ID (NCBI) 55658
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV68
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).