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验证数据展示

  • WB analysis of THP-1 using 86568-2-RR (same clone as 86568-2-PBS)

    WB analysis of THP-1 using 86568-2-RR (same clone as 86568-2-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 86568-2-RR (RIPK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86568-2-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 86568-2-RR (same clone as 86568-2-PBS)

    IF Staining of THP-1 using 86568-2-RR (same clone as 86568-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RIPK3 antibody (86568-2-RR, Clone: 251363G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86568-2-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 86568-2-RR (same clone as 86568-2-PBS)

    IHC staining of human placenta using 86568-2-RR (same clone as 86568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86568-2-RR (RIPK3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86568-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RIPK3 antibody included
  • Affinity and Kinetic Characterization of 86568-2-RR

    Affinity and Kinetic Characterization of 86568-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86568-2-RR against Human RIPK3 were performed. The affinity constant is 1.01 nM.

RIPK3 Recombinant monoclonal antibody, PBS Only

RIPK3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86568-2-PBS

产品说明书

CloneNo. 251363G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RIP3, EC:2.7.11.1, Receptor interacting protein 3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RIPK3 Ready-To-Use IHC Kit. RIPK3 primary antibody included.

产品信息

86568-2-PBS targets RIPK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30337

Product name: Recombinant human RIPK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-202 aa of BC062584

Sequence: HDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 receptor-interacting serine-threonine kinase 3
别名 RIP3, EC:2.7.11.1, Receptor interacting protein 3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3
计算分子量 518 aa, 57 kDa
观测分子量57 kDa
GenBank蛋白编号BC062584
基因名称 RIPK3
Gene ID (NCBI) 11035
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y572
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIPK3, also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).

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