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验证数据展示

  • IF Staining of HEK-293 using CL488-30119

    IF Staining of HEK-293 using CL488-30119

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 RIF1 antibody (CL488-30119) at dilution of 1:200.

CoraLite® Plus 488-conjugated RIF1 Polyclonal antibody

RIF1 Polyclonal Antibody for IF/ICC
Cat No. CL488-30119

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IF/ICC

别名

DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RIF1 Ready-To-Use IHC Kit. RIF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30119-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30119 targets RIF1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原

CatNo: Ag32422

Product name: Recombinant human RIF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2277-2472 aa of NM_001177663.1

Sequence: SEMAKESIPCPTESVYPPLVNCVAPVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDISEKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSCMANSVIKNLQSRWRSPSHENSI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAP1 interacting factor homolog (yeast)
别名 DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1
计算分子量274 kDa
观测分子量300 kDa
GenBank蛋白编号NM_001177663.1
基因名称 RIF1
Gene ID (NCBI) 55183
RRIDAB_3084125
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5UIP0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RIF1 antibody CL488-30119Download protocol
Standard Protocols
Click here to view our Standard Protocols
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