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验证数据展示

  • WB analysis using 30119-1-AP

    WB analysis using 30119-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30119-1-AP (RIF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 30119-1-AP

    IHC staining of human cervical cancer using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RIF1 antibody included
  • IHC staining of human cervical cancer using 30119-1-AP

    IHC staining of human cervical cancer using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RIF1 antibody included
  • IF Staining of HeLa using 30119-1-AP

    IF Staining of HeLa using 30119-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RIF1 antibody (30119-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse testis using 30119-1-AP

    IHC staining of mouse testis using 30119-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RIF1 antibody included

RIF1 Polyclonal antibody

RIF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30119-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HEK-293T cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RIF1 Ready-To-Use IHC Kit. RIF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30119-1-AP targets RIF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag32422

Product name: Recombinant human RIF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2277-2472 aa of NM_001177663.1

Sequence: SEMAKESIPCPTESVYPPLVNCVAPVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDISEKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSCMANSVIKNLQSRWRSPSHENSI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAP1 interacting factor homolog (yeast)
别名 DKFZp434D1026, DKFZp781N1478, FLJ12870, RIF1
计算分子量274 kDa
观测分子量300 kDa
GenBank蛋白编号NM_001177663.1
基因名称 RIF1
Gene ID (NCBI) 55183
RRIDAB_2935516
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5UIP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

实验方案

Product Specific Protocols
IF protocol for RIF1 antibody 30119-1-APDownload protocol
IHC protocol for RIF1 antibody 30119-1-APDownload protocol
WB protocol for RIF1 antibody 30119-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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