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RELL1 Polyclonal antibody

RELL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29823-1-AP

Print datasheet

Synonyms

FLJ21778, RELL1, RELT like 1, RELT like protein 1

验证数据展示

  • WB analysis of MCF-7 using 29823-1-AP

    WB analysis of MCF-7 using 29823-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 29823-1-AP (RELL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 29823-1-AP

    IHC staining of rat skin using 29823-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 29823-1-AP (RELL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29823-1-AP targets RELL1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Rat
Immunogen RELL1 fusion protein Ag31475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RELT-like 1
Synonyms FLJ21778, RELL1, RELT like 1, RELT like protein 1
Observed Molecular Weight29 kDa
GenBank Accession NumberBC039540
Gene Symbol RELL1
Gene ID (NCBI) 768211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELT-like protein 1 (RELL1) is a member of the tumor necrosis factor (TNF) receptor family and belongs to the receptor expressed in the lymphoid tissues (RELT) family. RELL1 activates the pro-inflammatory pathway by binding to TNF receptor-related factor 1 (TRAF1). Further, RELT family members activate p38 and induce apoptosis by a mechanism distinct from that of TNF receptors (PMID: 32090861). The molecular weight of RELL1 is 29 kDa.

实验方案

Product Specific Protocols
WB protocol for RELL1 antibody 29823-1-APDownload protocol
IHC protocol for RELL1 antibody 29823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols