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CoraLite® Plus 647-conjugated RELB Polyclonal antibody

RELB Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-25027

Print datasheet

Synonyms

I REL, IREL, RELB, Transcription factor RelB

验证数据展示

  • FC experiment of HeLa using CL647-25027

    FC experiment of HeLa using CL647-25027

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human RELB (CL647-25027) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25027-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-25027 is RELB Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen RELB fusion protein Ag13824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms I REL, IREL, RELB, Transcription factor RelB
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028013
Gene Symbol RELB
Gene ID (NCBI) 5971
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01201
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 RELB antibody CL647-25027Download protocol
Standard Protocols
Click here to view our Standard Protocols