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CoraLite® Plus 488-conjugated RELB Polyclonal antibody

RELB Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-25027

Print datasheet

Synonyms

I REL, IREL, RELB, Transcription factor RelB

验证数据展示

  • IF Staining of HeLa using CL488-25027

    IF Staining of HeLa using CL488-25027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RELB antibody (CL488-25027) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-25027

    FC experiment of Jurkat using CL488-25027

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human RELB (CL488-25027) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25027-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-25027 is RELB Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen RELB fusion protein Ag13824 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms I REL, IREL, RELB, Transcription factor RelB
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028013
Gene Symbol RELB
Gene ID (NCBI) 5971
RRIDAB_2919201
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01201
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RELB antibody CL488-25027Download protocol
FC protocol for CL Plus 488 RELB antibody CL488-25027Download protocol
Standard Protocols
Click here to view our Standard Protocols