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验证数据展示

  • WB analysis using 68119-1-Ig

    WB analysis using 68119-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 68119-1-Ig

    WB analysis of HeLa using 68119-1-Ig

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells.

  • IHC staining of human colon cancer using 68119-1-Ig

    IHC staining of human colon cancer using 68119-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 68119-1-Ig

    IF Staining of A549 using 68119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • WB analysis of pig heart using 68119-1-Ig

    WB analysis of pig heart using 68119-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

REEP5 Monoclonal antibody

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68119-1-Ig

产品说明书

CloneNo. 1H8D8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

1H8D8, C5orf18, DP1, Polyposis locus protein 1, Protein TB2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, pig heart tissue, HeLa cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68119-1-Ig targets REEP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag6229

Product name: Recombinant human REEP5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-189 aa of BC065926

Sequence: MSAAMRERFDRFLHEKNCMTDLLAKLEAKTGVNRSFIALGVIGLVALYLVFGYGASLLCNLIGFGYPAYISIKAIESPNKEDDTQWLTYWVVYGVFSIAEFFSDIFLSWFPFYYMLKCGFLLWCMAPSPSNGAELLYKRIIRPFFLKHESQMDSVVKDLKDKAKETADAITKEAKKATVNLLGEEKKST

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 receptor accessory protein 5
别名 1H8D8, C5orf18, DP1, Polyposis locus protein 1, Protein TB2
计算分子量 21 kDa
观测分子量18 kDa
GenBank蛋白编号BC065926
基因名称 REEP5
Gene ID (NCBI) 7905
RRIDAB_2923648
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ00765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

实验方案

Product Specific Protocols
IF protocol for REEP5 antibody 68119-1-IgDownload protocol
IHC protocol for REEP5 antibody 68119-1-IgDownload protocol
WB protocol for REEP5 antibody 68119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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