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验证数据展示

  • WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis using 68119-1-Ig (same clone as 68119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis of HeLa using 68119-1-Ig (same clone as 68119-1-PBS)

    WB result of REEP5 antibody (68119-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REEP5 transfected HeLa cells. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

    IHC staining of human colon cancer using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68119-1-Ig (REEP5 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

    IF Staining of A549 using 68119-1-Ig (same clone as 68119-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using REEP5 antibody (68119-1-Ig, Clone: 1H8D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

    WB analysis of pig heart using 68119-1-Ig (same clone as 68119-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 68119-1-Ig (REEP5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68119-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1

    Cytometric bead array standard curve of MP50780-1, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-2-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2

    Cytometric bead array standard curve of MP50780-2, REEP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68119-1-PBS. Detection antibody: 68119-3-PBS. Standard:Ag6229. Range: 0.098-100 ng/mL.

  • Featured Product
  • KD/KO验证

REEP5 Monoclonal antibody, PBS Only (Capture)

REEP5 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68119-1-PBS

产品说明书

CloneNo. 1H8D8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1H8D8, C5orf18, DP1, Polyposis locus protein 1, Protein TB2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68119-1-PBS targets REEP5 as part of a matched antibody pair:

MP50780-1: 68119-1-PBS capture and 68119-2-PBS detection (validated in Cytometric bead array)

MP50780-2: 68119-1-PBS capture and 68119-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag6229

Product name: Recombinant human REEP5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-189 aa of BC065926

Sequence: MSAAMRERFDRFLHEKNCMTDLLAKLEAKTGVNRSFIALGVIGLVALYLVFGYGASLLCNLIGFGYPAYISIKAIESPNKEDDTQWLTYWVVYGVFSIAEFFSDIFLSWFPFYYMLKCGFLLWCMAPSPSNGAELLYKRIIRPFFLKHESQMDSVVKDLKDKAKETADAITKEAKKATVNLLGEEKKST

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 receptor accessory protein 5
别名 1H8D8, C5orf18, DP1, Polyposis locus protein 1, Protein TB2
计算分子量 21 kDa
观测分子量18 kDa
GenBank蛋白编号BC065926
基因名称 REEP5
Gene ID (NCBI) 7905
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ00765
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

REEP5 (receptor expression-enhancing protein 5), also named as C5orf18, DP1 or TB2, is a membrane protein belonging to DP1/Yop1p protein family. All REEP proteins contain a conserved TB2/DP1, HVA22 domain which is involved in intracellular trafficking and secretion (PMID: 19123125). REEP5 is expressed in circumvallate papillae and testis, and may have the function to influence functional cell surface expression of taste receptors (PMID: 16720576). REEP5 can interact with atlastins (ATL1 and ATL2) which are involved in modulating the structure of ER network (PMID: 19665976).

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