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验证数据展示

  • WB analysis using 83363-1-RR

    WB analysis using 83363-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 83363-1-RR

    FC experiment of K-562 using 83363-1-RR

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83363-1-RR

    Affinity and Kinetic Characterization of 83363-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

RBM20 Recombinant monoclonal antibody

RBM20 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83363-1-RR

产品说明书

CloneNo. 240345B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

RNA binding protein, RNA binding motif protein 20, 240345B7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, K-562 cells, TT cells, A549 cells
Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83363-1-RR targets RBM20 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34322

Product name: Recombinant human RBM20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 667-840 aa of NM_001134363

Sequence: ADWGNGRDSWEHSPYARREEERDPAPWRDNGDDKRDRMDPWAHDRKHHPRQLDKAELDERPEGGRPHREKYPRSGSPNLPHSVSSYKSREDGYYRKEPKAKSDKYLKQQQDAPGRSRRKDEARLRESRHPHPDDSGKEDGLGPKVTRAPEGAKAKQNEKNKTKRTDRDQEGADD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 20
别名 RNA binding protein, RNA binding motif protein 20, 240345B7
计算分子量134 kDa
观测分子量190 kDa
GenBank蛋白编号NM_001134363
基因名称 RBM20
Gene ID (NCBI) 282996
RRIDAB_3671017
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ5T481
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).

实验方案

Product Specific Protocols
WB protocol for RBM20 antibody 83363-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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