• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HeLa using CL488-18893

    IF Staining of HeLa using CL488-18893

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RBM16 antibody (CL488-18893) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-18893

    FC experiment of HeLa using CL488-18893

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody (CL488-18893)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-18893

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

RBM16, RNA binding protein 16, RNA-binding motif protein 16, SCAF8, SR-related and CTD-associated factor 8, CCAP7, CDC5L complex-associated protein 7, KIAA1116

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SCAF8 Ready-To-Use IHC Kit. SCAF8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 18893-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18893 targets RBM16 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 RBM16 fusion protein Ag13499 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 16
别名 RBM16, RNA binding protein 16, RNA-binding motif protein 16, SCAF8, SR-related and CTD-associated factor 8, CCAP7, CDC5L complex-associated protein 7, KIAA1116
计算分子量 1271 aa, 141 kDa
观测分子量 170 kDa
GenBank蛋白编号BC070071
基因名称 RBM16
Gene ID (NCBI) 22828
RRIDAB_3672703
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
FC protocol for CL Plus 488 RBM16 antibody CL488-18893Download protocol
Standard Protocols
Click here to view our Standard Protocols