• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HT-1080 using 18893-1-AP

    WB analysis of HT-1080 using 18893-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18893-1-AP

    IHC staining of human testis using 18893-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SCAF8 antibody included
  • IP experiment of HeLa using 18893-1-AP

    IP experiment of HeLa using 18893-1-AP

    IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug.

  • WB analysis using 18893-1-AP

    WB analysis using 18893-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18893-1-AP

    IF Staining of HeLa using 18893-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

RBM16 Polyclonal antibody

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18893-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

SCAF8, RNA-binding motif protein 16, RNA binding protein 16, KIAA1116, CDC5L complex-associated protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, L02 cells, HT-1080 cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SCAF8 Ready-To-Use IHC Kit. SCAF8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18893-1-AP targets RBM16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 RBM16 fusion protein Ag13499 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 16
别名 SCAF8, RNA-binding motif protein 16, RNA binding protein 16, KIAA1116, CDC5L complex-associated protein 7
计算分子量 1271 aa, 141 kDa
观测分子量 170 kDa
GenBank蛋白编号BC070071
基因名称 RBM16
Gene ID (NCBI) 22828
RRIDAB_2175908
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

实验方案

Product Specific Protocols
WB protocol for RBM16 antibody 18893-1-APDownload protocol
IHC protocol for RBM16 antibody 18893-1-APDownload protocol
IF protocol for RBM16 antibody 18893-1-APDownload protocol
IP protocol for RBM16 antibody 18893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols