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CoraLite®594-conjugated RBAP48 Monoclonal antibody

RBAP48 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5C4D6

Cat No : CL594-66060

Print datasheet

Synonyms

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

验证数据展示

  • FC experiment of HepG2 using CL594-66060

    FC experiment of HepG2 using CL594-66060

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of A549 using CL594-66060

    IF Staining of A549 using CL594-66060

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594-conjugated RBAP48 antibody (CL594-66060, Clone: 5C4D6 ) at dilution of 1:200, CL488-Phalloidin (green).

经过测试的应用

Positive IF detected inA549 cells
Positive FC detected inHepG2 cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy RBBP4 Ready-To-Use IHC Kit. RBBP4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66060-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66060 is RBAP48 Fusion Protein expressed in E. coli.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen RBAP48 fusion protein Ag6196 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 4
Synonyms CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC053904
Gene Symbol RBBP4
Gene ID (NCBI) 5928
RRIDAB_2883497
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09028
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

实验方案

Product Specific Protocols
IF protocol for CL594 RBAP48 antibody CL594-66060Download protocol
FC protocol for CL594 RBAP48 antibody CL594-66060Download protocol
Standard Protocols
Click here to view our Standard Protocols