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CoraLite® Plus 488-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10C3

Cat No : CL488-68011

Print datasheet

Synonyms

Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly

验证数据展示

  • IF Staining of A431 using CL488-68011

    IF Staining of A431 using CL488-68011

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RALY antibody (CL488-68011, Clone: 2B10C3 ) at dilution of 1:600.

  • FC experiment of A431 using CL488-68011

    FC experiment of A431 using CL488-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of A431 using CL488-68011

    FC experiment of A431 using CL488-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 68011-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68011 targets RALY in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen RALY fusion protein Ag12250 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Synonyms Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly
Calculated Molecular Weight 307 aa, 33 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC103753
Gene Symbol RALY
Gene ID (NCBI) 22913
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UKM9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RALY antibody CL488-68011Download protocol
FC protocol for CL Plus 488 RALY antibody CL488-68011Download protocol
Standard Protocols
Click here to view our Standard Protocols