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验证数据展示

  • WB analysis of NIH/3T3 using 67359-1-Ig

    WB analysis of NIH/3T3 using 67359-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 67359-1-Ig

    FC experiment of HEK-293 using 67359-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug RALBP1 Monoclonal antibody (67359-1-Ig, Clone:1B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67359-1-Ig

产品说明书

CloneNo. 1B2F5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

1B2F5, 76 kDa Ral interacting protein, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, DNP SG ATPase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67359-1-Ig targets RALBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28618

Product name: Recombinant human RALBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-351 aa of BC013126

Sequence: MTECFLPPTSSPSEHRRVEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDILHEPPDVVSDDEKDHGKKKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKMKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEKHKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQIDVPNLKPIFGIPLADAVERTMMYDGIRLPAVFRECIDYVEKYGMKCEGIYRVSGIKSKVDELKAAYDREESTNLEDYEPNTVASLLKQYLRDLPENLLTKELMPRFEEACGRTTETEKVQEFQRLLKELPECNYLLISWLIVHMDHVIAKELETKMNIQNISIVLSPTVQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ralA binding protein 1
别名 1B2F5, 76 kDa Ral interacting protein, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, DNP SG ATPase
计算分子量 655 aa, 76 kDa
观测分子量 95 kDa
GenBank蛋白编号BC013126
基因名称 RALBP1
Gene ID (NCBI) 10928
RRIDAB_2882611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for RALBP1 antibody 67359-1-IgDownload protocol
WB protocol for RALBP1 antibody 67359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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