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RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1B2F5

Cat No : 67359-1-Ig

Print datasheet

Synonyms

76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76

验证数据展示

  • WB analysis of NIH/3T3 using 67359-1-Ig

    WB analysis of NIH/3T3 using 67359-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 67359-1-Ig

    FC experiment of HEK-293 using 67359-1-Ig

    1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human RALBP1 (67359-1-Ig, Clone:1B2F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

经过测试的应用

Positive WB detected inNIH/3T3 cells, Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67359-1-Ig targets RALBP1 in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, WB,ELISA
Tested Reactivity Human, mouse
Immunogen RALBP1 fusion protein Ag28618 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ralA binding protein 1
Synonyms 76 kDa Ral interacting protein, DNP SG ATPase, Ral interacting protein 1, ralA binding protein 1, RALBP1, RIP1, RLIP1, RLIP76
Calculated Molecular Weight 655 aa, 76 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC013126
Gene Symbol RALBP1
Gene ID (NCBI) 10928
RRIDAB_2882611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15311
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALBP1 antibody 67359-1-IgDownload protocol
FC protocol for RALBP1 antibody 67359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols