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验证数据展示

  • WB analysis using 86581-1-RR (same clone as 86581-1-PBS)

    WB analysis using 86581-1-RR (same clone as 86581-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

  • WB analysis of rat testis using 86581-1-RR (same clone as 86581-1-PBS)

    WB analysis of rat testis using 86581-1-RR (same clone as 86581-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86581-1-RR (same clone as 86581-1-PBS)

    IF Staining of HeLa using 86581-1-RR (same clone as 86581-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAI14 antibody (86581-1-RR, Clone: 251495G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86581-1-RR

    Affinity and Kinetic Characterization of 86581-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86581-1-RR against Human RAI14 were performed. The affinity constant is 7.73 nM.

RAI14 Recombinant monoclonal antibody, PBS Only

RAI14 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86581-1-PBS

产品说明书

CloneNo. 251495G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86581-1-PBS targets RAI14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11708

Product name: Recombinant human RAI14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-347 aa of BC052988

Sequence: MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGKTALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNSRNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLSGKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 retinoic acid induced 14
别名 Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13
计算分子量 980 aa, 110 kDa
观测分子量 110 kDa
GenBank蛋白编号BC052988
基因名称 RAI14
Gene ID (NCBI) 26064
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P0K7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.

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