验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat testis tissue, HEK-293T cells, NIH/3T3 cells, mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86581-1-RR targets RAI14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11708 Product name: Recombinant human RAI14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC052988 Sequence: MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLAAAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGKTALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNSRNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSKISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLSGKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | retinoic acid induced 14 |
| 别名 | Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13 |
| 计算分子量 | 980 aa, 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC052988 |
| 基因名称 | RAI14 |
| Gene ID (NCBI) | 26064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9P0K7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RAI14 antibody 86581-1-RR | Download protocol |
| WB protocol for RAI14 antibody 86581-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
