验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84994-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84994-4 targets RAF1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RAF1 fusion protein Ag25423 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-raf-1 murine leukemia viral oncogene homolog 1 |
| 别名 | 242540A2, c Raf, CRAF, EC:2.7.11.1, NS5 |
| 计算分子量 | 648 aa, 73 kDa |
| 观测分子量 | ~75 kDa |
| GenBank蛋白编号 | BC018119 |
| 基因名称 | RAF1 |
| Gene ID (NCBI) | 5894 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04049 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAF1 antibody CL488-84994-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |