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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84994-4-RR (same clone as 84994-4-PBS)

    WB analysis using 84994-4-RR (same clone as 84994-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84994-4-RR (same clone as 84994-4-PBS)

    IF Staining of HeLa using 84994-4-RR (same clone as 84994-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84994-4-RR

    Affinity and Kinetic Characterization of 84994-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

RAF1 Recombinant antibody, PBS Only

RAF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84994-4-PBS

产品说明书

CloneNo. 242540A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.

产品信息

84994-4-PBS targets RAF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAF1 fusion protein Ag25423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-raf-1 murine leukemia viral oncogene homolog 1
别名 Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf
计算分子量 648 aa, 73 kDa
观测分子量~75 kDa
GenBank蛋白编号BC018119
基因名称 RAF1
Gene ID (NCBI) 5894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04049
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.