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验证数据展示

  • WB analysis using 84994-4-RR

    WB analysis using 84994-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84994-4-RR

    IF Staining of HeLa using 84994-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84994-4-RR

    Affinity and Kinetic Characterization of 84994-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

RAF1 Recombinant antibody

RAF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84994-4-RR

产品说明书

CloneNo. 242540A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, HeLa cells, DU 145 cells, NIH/3T3 cells, PC-12 cells, RAW 264.7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAF1 Ready-To-Use IHC Kit. RAF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84994-4-RR targets RAF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAF1 fusion protein Ag25423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-raf-1 murine leukemia viral oncogene homolog 1
别名 Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf
计算分子量 648 aa, 73 kDa
观测分子量~75 kDa
GenBank蛋白编号BC018119
基因名称 RAF1
Gene ID (NCBI) 5894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04049
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

实验方案

Product Specific Protocols
WB protocol for RAF1 antibody 84994-4-RRDownload protocol
IF protocol for RAF1 antibody 84994-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols