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验证数据展示

  • WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83712-1-PBS

    Affinity and Kinetic Characterization of 83712-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 83712-1-PBS against Human RAE1 was performed. The affinity constant is 1.14 nM.

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

RAE1 Recombinant monoclonal antibody, PBS Only

RAE1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83712-1-PBS

产品说明书

CloneNo. 240861E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

产品信息

83712-1-PBS targets RAE1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12102

Product name: Recombinant human RAE1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC103754

Sequence: MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAE1 RNA export 1 homolog (S. pombe)
别名 240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1
计算分子量 368 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC103754
基因名称 RAE1
Gene ID (NCBI) 8480
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78406
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

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