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验证数据展示

  • WB analysis using 83712-1-RR

    WB analysis using 83712-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83712-1-RR

    FC experiment of HepG2 using 83712-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83712-1-RR

    Affinity and Kinetic Characterization of 83712-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

RAE1 Recombinant monoclonal antibody

RAE1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83712-1-RR

产品说明书

CloneNo. 240861E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse brain tissue, mouse thymus tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RAE1 Ready-To-Use IHC Kit. RAE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83712-1-RR targets RAE1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12102

Product name: Recombinant human RAE1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC103754

Sequence: MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAE1 RNA export 1 homolog (S. pombe)
别名 240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1
计算分子量 368 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC103754
基因名称 RAE1
Gene ID (NCBI) 8480
RRIDAB_3671315
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78406
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

实验方案

Product Specific Protocols
FC protocol for RAE1 antibody 83712-1-RRDownload protocol
WB protocol for RAE1 antibody 83712-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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