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验证数据展示

  • WB analysis using 84969-4-RR

    WB analysis using 84969-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84969-4-RR (RAD50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 84969-4-RR

    WB analysis of MCF-7 using 84969-4-RR

    WB result of RAD50 antibody (84969-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD50 transfected MCF-7 cells.

  • IHC staining of human placenta using 84969-4-RR

    IHC staining of human placenta using 84969-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAD50 antibody included
  • IHC staining of human placenta using 84969-4-RR

    IHC staining of human placenta using 84969-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAD50 antibody included
  • Affinity and Kinetic Characterization of 84969-4-RR

    Affinity and Kinetic Characterization of 84969-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84969-4-RR against Human RAD50 were performed. The affinity constant is 6.79 nM.

  • Featured Product
  • KD/KO验证

RAD50 Recombinant monoclonal antibody

RAD50 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84969-4-RR

产品说明书

CloneNo. 242541A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242541A5, hRad50, RAD50 homolog (S. cerevisiae)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, Jurkat cells, MOLT-4 cells, C6 cells
Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, mouse brain tissue, mouse lung tissue, mouse stomach tissue, rat brain tissue, rat lung tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAD50 Ready-To-Use IHC Kit. RAD50 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84969-4-RR targets RAD50 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29949

Product name: Recombinant human RAD50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 275-425 aa of NM_005732

Sequence: LDSRKKQMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQGRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAEKETLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAD50 homolog (S. cerevisiae)
别名 242541A5, hRad50, RAD50 homolog (S. cerevisiae)
计算分子量 154 kDa
观测分子量 154 kDa
GenBank蛋白编号NM_005732
基因名称 RAD50
Gene ID (NCBI) 10111
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92878
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

实验方案

Product Specific Protocols
IHC protocol for RAD50 antibody 84969-4-RRDownload protocol
WB protocol for RAD50 antibody 84969-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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