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验证数据展示

  • WB analysis using 84969-4-RR

    WB analysis using 84969-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84969-4-RR (RAD50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 84969-4-RR

    IHC staining of human placenta using 84969-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 84969-4-RR

    IHC staining of human placenta using 84969-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84969-4-RR

    Affinity and Kinetic Characterization of 84969-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84969-4-RR against Human RAD50 were performed. The affinity constant is 6.79 nM.

RAD50 Recombinant antibody

RAD50 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84969-4-RR

产品说明书

CloneNo. 242541A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

242541A5, hRad50, RAD50 homolog (S. cerevisiae)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, Jurkat cells, MOLT-4 cells, C6 cells
Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, mouse brain tissue, mouse lung tissue, mouse stomach tissue, rat brain tissue, rat lung tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84969-4-RR targets RAD50 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAD50 fusion protein Ag29949 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAD50 homolog (S. cerevisiae)
别名 242541A5, hRad50, RAD50 homolog (S. cerevisiae)
计算分子量 154 kDa
观测分子量 154 kDa
GenBank蛋白编号NM_005732
基因名称 RAD50
Gene ID (NCBI) 10111
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92878
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

实验方案

Product Specific Protocols
WB protocol for RAD50 antibody 84969-4-RRDownload protocol
IHC protocol for RAD50 antibody 84969-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols