验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RAC1 Ready-To-Use IHC Kit. RAC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66122-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-66122 targets RAC1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | RAC1 fusion protein Ag17779 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) |
| 别名 | MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25 |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC004247 |
| 基因名称 | RAC1 |
| Gene ID (NCBI) | 5879 |
| RRID | AB_2883264 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P63000 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RAC1 antibody CL488-66122 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
