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CoraLite® Plus 488-conjugated RAB39B Monoclonal antibody

RAB39B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F4F7

Cat No : CL488-68014

Print datasheet

Synonyms

RAB39B, Ras related protein Rab 39B

验证数据展示

  • FC experiment of SH-SY5Y using CL488-68014

    FC experiment of SH-SY5Y using CL488-68014

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB39B (CL488-68014, Clone:2F4F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 68014-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68014 targets RAB39B in FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen RAB39B fusion protein Ag27170 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name RAB39B, member RAS oncogene family
Synonyms RAB39B, Ras related protein Rab 39B
Calculated Molecular Weight 213 aa, 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC009714
Gene Symbol RAB39B
Gene ID (NCBI) 116442
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96DA2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB39B is a member of the RAB GTPase family with a putative role in vesicle trafficking. RAB39B is predominantly expressed in neuronal cells and is localized to the endoplasmic reticulum, Golgi and trans‐Golgi recycling endosomes. Several reports suggested that RAB39B may regulate protein trafficking and the PI3K-AKT-mTOR pathway. Mutations in RAB39B are known to be associated with X-linked intellectual disability (XLID), Parkinson's disease, and autism.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RAB39B antibody CL488-68014Download protocol
Standard Protocols
Click here to view our Standard Protocols