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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68389-1-Ig (same clone as 68389-1-PBS)

    WB analysis using 68389-1-Ig (same clone as 68389-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 68389-1-Ig (same clone as 68389-1-PBS)

    WB analysis of SH-SY5Y using 68389-1-Ig (same clone as 68389-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68389-1-Ig (RAB33A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 68389-1-Ig (same clone as 68389-1-PBS)

    IF Staining of Neuro-2a using 68389-1-Ig (same clone as 68389-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using RAB33A antibody (68389-1-Ig, Clone: 1C7F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68389-1-PBS in a different storage buffer formulation.

RAB33A Monoclonal antibody, PBS Only

RAB33A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68389-1-PBS

产品说明书

CloneNo. 1C7F11

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse, pig, rabbit

应用

WB, IF/ICC, Indirect ELISA

别名

RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68389-1-PBS targets RAB33A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse, pig, rabbit
免疫原 RAB33A fusion protein Ag33188 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB33A, member RAS oncogene family
别名 RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10
计算分子量 27 kDa
GenBank蛋白编号BC009996
基因名称 RAB33A
Gene ID (NCBI) 9363
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14088
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).