验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, SH-SY5Y cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
68389-1-Ig targets RAB33A in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, rat, mouse, pig, rabbit |
| 文献引用反应性 | mouse |
| 免疫原 | RAB33A fusion protein Ag33188 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAB33A, member RAS oncogene family |
| 别名 | RAB33A, RabS10, Ras related protein Rab 33A, Small GTP binding protein S10 |
| 计算分子量 | 27 kDa |
| GenBank蛋白编号 | BC009996 |
| 基因名称 | RAB33A |
| Gene ID (NCBI) | 9363 |
| RRID | AB_3085107 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14088 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ras-related protein Rab-33A (RAB33A) is also named as RABS10, and belongs to the small GTPase superfamily and the Rab family. RAB33A is an X-linked gene that is expressed in brain, lymphocytes, and normal melanocytes, but is downregulated in melanoma cells. In the brain, RAB33A is present throughout the cortex, as well as in the hippocampal CA fields (PMID:16810302). RAB33A is expressed in cells, including neurons, lymphocytes, melanocytes and parotid acinar cells (PMID:16810302, PMID:25871792). In cultured rat hippocampal neurons, RAB33A is localized to the Golgi apparatus and post-Golgi vesicles transported along axons (PMID:22972995).In addition, RAB33A interacts with singar1/RUFY3, which suppresses the formation of surplus axons in cultured hippocampal neurons (PMID:21737958, PMID:17439943).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB33A antibody 68389-1-Ig | Download protocol |
| IF protocol for RAB33A antibody 68389-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |