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验证数据展示

  • WB analysis using CL488-82960

    WB analysis using CL488-82960

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-82960 (RAB32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL488-82960

    FC experiment of HeLa using CL488-82960

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rab32 Recombinant Antibody (CL488-82960, Clone:230315A9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RAB32 Recombinant monoclonal antibody

RAB32 Uni-rAb® Recombinant Antibody for WB, FC (Intra)
Cat No. CL488-82960

产品说明书

CloneNo. 230315A9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra)

别名

230315A9, Ras related protein Rab 32, Ras-related protein Rab-32

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82960-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82960 targets RAB32 in WB, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag1450

Product name: Recombinant human RAB32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC015061

Sequence: MAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB32, member RAS oncogene family
别名 230315A9, Ras related protein Rab 32, Ras-related protein Rab-32
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC015061
基因名称 RAB32
Gene ID (NCBI) 10981
RRIDAB_3673152
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13637
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 RAB32 antibody CL488-82960Download protocol
WB protocol for CL Plus 488 RAB32 antibody CL488-82960Download protocol
Standard Protocols
Click here to view our Standard Protocols
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