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验证数据展示

  • WB analysis using 82960-1-RR (same clone as 82960-1-PBS)

    WB analysis using 82960-1-RR (same clone as 82960-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82960-1-RR (RAB32 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82960-1-RR (same clone as 82960-1-PBS)

    IF Staining of HeLa using 82960-1-RR (same clone as 82960-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-1-RR, Clone: 230315A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 82960-1-RR (same clone as 82960-1-PBS)

    FC experiment of U-251 using 82960-1-RR (same clone as 82960-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human RAB32 (82960-1-RR, Clone:230315A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82960-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82960-1-RR

    Affinity and Kinetic Characterization of 82960-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82960-1-RR against Human RAB32 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

RAB32 Recombinant monoclonal antibody, PBS Only

RAB32 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82960-1-PBS

产品说明书

CloneNo. 230315A9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

230315A9, Ras related protein Rab 32, Ras-related protein Rab-32

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAB32 Ready-To-Use IHC Kit. RAB32 primary antibody included.

产品信息

82960-1-PBS targets RAB32 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag1450

Product name: Recombinant human RAB32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC015061

Sequence: MAGGGAGDPGLGAAAAPAPETREHLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSRTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDISRSSTFEAVLKWKSDLDSKVHLPNGSPIPAVLLANKCDQNKDSSQSPSQVDQFCKEHGFAGWFETSAKDNINIEEAARFLVEKILVNHQSFPNEENDVDKIKLDQETLRAENKSQCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB32, member RAS oncogene family
别名 230315A9, Ras related protein Rab 32, Ras-related protein Rab-32
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC015061
基因名称 RAB32
Gene ID (NCBI) 10981
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13637
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

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