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CoraLite®594-conjugated RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C2G6

Cat No : CL594-67837

Print datasheet

Synonyms

RAB1B, Ras related protein Rab 1B

验证数据展示

  • FC experiment of NIH/3T3 using CL594-67837

    FC experiment of NIH/3T3 using CL594-67837

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB1B (CL594-67837, Clone:3C2G6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using CL594-67837

    IF Staining of NIH/3T3 using CL594-67837

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite®594 RAB1B antibody (CL594-67837, Clone: 3C2G6 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67837-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67837 targets RAB1B in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen RAB1B fusion protein Ag12069 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB1B, member RAS oncogene family
Synonyms RAB1B, Ras related protein Rab 1B
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H0U4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB1B antibody CL594-67837Download protocol
FC protocol for CL594 RAB1B antibody CL594-67837Download protocol
Standard Protocols
Click here to view our Standard Protocols