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验证数据展示

  • WB analysis using 67837-1-Ig

    WB analysis using 67837-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67837-1-Ig

    WB analysis of NIH/3T3 using 67837-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67837-1-Ig

    WB analysis of HeLa using 67837-1-Ig

    WB result of RAB1B antibody (67837-1-Ig; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

  • IHC staining of human lung cancer using 67837-1-Ig

    IHC staining of human lung cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human lung cancer using 67837-1-Ig

    IHC staining of human lung cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human pancreas cancer using 67837-1-Ig

    IHC staining of human pancreas cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human pancreas cancer using 67837-1-Ig

    IHC staining of human pancreas cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of rat heart using 67837-1-Ig

    IHC staining of rat heart using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of rat heart using 67837-1-Ig

    IHC staining of rat heart using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human liver cancer using 67837-1-Ig

    IHC staining of human liver cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human stomach cancer using 67837-1-Ig

    IHC staining of human stomach cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human stomach cancer using 67837-1-Ig

    IHC staining of human stomach cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of rat brain using 67837-1-Ig

    IHC staining of rat brain using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of rat brain using 67837-1-Ig

    IHC staining of rat brain using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IHC staining of human ovary tumor using 67837-1-Ig

    IHC staining of human ovary tumor using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB1B antibody included
  • IF Staining of MCF-7 using 67837-1-Ig

    IF Staining of MCF-7 using 67837-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of NIH/3T3 using 67837-1-Ig

    IF Staining of NIH/3T3 using 67837-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67837-1-Ig

产品说明书

CloneNo. 3C2G6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

3C2G6, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human stomach cancer tissue, rat brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1B Ready-To-Use IHC Kit. RAB1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67837-1-Ig targets RAB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12069

Product name: Recombinant human RAB1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-201 aa of BC071169

Sequence: MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB1B, member RAS oncogene family
别名 3C2G6, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B
计算分子量 201 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC071169
基因名称 RAB1B
Gene ID (NCBI) 81876
RRIDAB_2918599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H0U4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RAB1B antibody 67837-1-IgDownload protocol
IHC protocol for RAB1B antibody 67837-1-IgDownload protocol
WB protocol for RAB1B antibody 67837-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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