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验证数据展示

  • IF Staining of NIH/3T3 using CL488-11671

    IF Staining of NIH/3T3 using CL488-11671

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 RAB1A antibody (CL488-11671) at dilution of 1:400.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated RAB1A Polyclonal antibody

RAB1A Polyclonal Antibody for IF/ICC
Cat No. CL488-11671

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

DKFZp564B163, RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy RAB1A Ready-To-Use IHC Kit. RAB1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11671-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11671 targets RAB1A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2264

Product name: Recombinant human RAB1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-205 aa of BC000905

Sequence: MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB1A, member RAS oncogene family
别名 DKFZp564B163, RAB1, RAB1A, Ras related protein Rab 1A, YPT1, YPT1 related protein
计算分子量 205 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC000905
基因名称 RAB1A
Gene ID (NCBI) 5861
RRIDAB_3672504
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62820
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab1A is a member of the Rab family of small GTPases with a well characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. Rab1A has recently been reported an mTORC1 activator and a colorectal oncogene (Janice D. Thomas. et al. 2014. Cancer cell).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAB1A antibody CL488-11671Download protocol
Standard Protocols
Click here to view our Standard Protocols
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