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验证数据展示

  • WB analysis using 31416-1-AP (same clone as 31416-1-PBS)

    WB analysis using 31416-1-AP (same clone as 31416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31416-1-AP (RAB13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 31416-1-AP (same clone as 31416-1-PBS)

    WB analysis of HEK-293 using 31416-1-AP (same clone as 31416-1-PBS)

    WB result of RAB13 antibody (31416-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31416-1-AP (same clone as 31416-1-PBS)

    IHC staining of mouse testis using 31416-1-AP (same clone as 31416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB13 antibody included
  • IHC staining of mouse stomach using 31416-1-AP (same clone as 31416-1-PBS)

    IHC staining of mouse stomach using 31416-1-AP (same clone as 31416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RAB13 antibody included
  • FC experiment of K-562 using 31416-1-AP (same clone as 31416-1-PBS)

    FC experiment of K-562 using 31416-1-AP (same clone as 31416-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug RAB13 Polyclonal antibody (31416-1-AP) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAB13 Polyclonal antibody, PBS Only

RAB13 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 31416-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

产品信息

31416-1-PBS targets RAB13 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34898

Product name: Recombinant human RAB13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-200 aa of BC000799

Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB13, member RAS oncogene family
别名 GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13
计算分子量 203 aa, 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC000799
基因名称 RAB13
Gene ID (NCBI) 5872
RRIDAB_3669972
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP51153
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

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