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验证数据展示

  • WB analysis using 31416-1-AP

    WB analysis using 31416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31416-1-AP (RAB13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31416-1-AP

    IHC staining of mouse testis using 31416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB13 antibody included
  • IHC staining of mouse stomach using 31416-1-AP

    IHC staining of mouse stomach using 31416-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB13 antibody included
  • FC experiment of K-562 using 31416-1-AP

    FC experiment of K-562 using 31416-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug RAB13 Polyclonal antibody (31416-1-AP) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RAB13 Polyclonal antibody

RAB13 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 31416-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31416-1-AP targets RAB13 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 RAB13 fusion protein Ag34898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB13, member RAS oncogene family
别名 GIG4, Ras-related protein Rab-13, Ras related protein Rab 13, Cell growth-inhibiting gene 4 protein, Cell growth inhibiting gene 4 protein
计算分子量 203 aa, 23 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC000799
基因名称 RAB13
Gene ID (NCBI) 5872
RRIDAB_3669972
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP51153
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

实验方案

Product Specific Protocols
WB protocol for RAB13 antibody 31416-1-APDownload protocol
IHC protocol for RAB13 antibody 31416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols