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验证数据展示

  • WB analysis using 30236-1-AP

    WB analysis using 30236-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 30236-1-AP

    WB analysis of Jurkat using 30236-1-AP

    WB result of QRICH1 antibody (30236-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-QRICH1 transfected Jurkat cells.

  • WB analysis using 30236-1-AP

    WB analysis using 30236-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 30236-1-AP

    IHC staining of human lung cancer using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of human lung cancer using 30236-1-AP

    IHC staining of human lung cancer using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of mouse testis using 30236-1-AP

    IHC staining of mouse testis using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of mouse testis using 30236-1-AP

    IHC staining of mouse testis using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of mouse brain using 30236-1-AP

    IHC staining of mouse brain using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of mouse brain using 30236-1-AP

    IHC staining of mouse brain using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of rat brain using 30236-1-AP

    IHC staining of rat brain using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of rat brain using 30236-1-AP

    IHC staining of rat brain using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of human colon cancer using 30236-1-AP

    IHC staining of human colon cancer using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IHC staining of human colon cancer using 30236-1-AP

    IHC staining of human colon cancer using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • IF Staining of U2OS using 30236-1-AP

    IF Staining of U2OS using 30236-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using QRICH1 antibody (30236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse stomach using 30236-1-AP

    IHC staining of mouse stomach using 30236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with QRICH1 antibody included
  • Featured Product
  • KD/KO验证

QRICH1 Polyclonal antibody

QRICH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30236-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Transcriptional regulator QRICH1, Glutamine-rich protein 1, Glutamine rich protein 1, glutamine rich 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, rat brain tissue, human colon cancer tissue, human lung cancer tissue, mouse brain tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy QRICH1 Ready-To-Use IHC Kit. QRICH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30236-1-AP targets QRICH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31997

Product name: Recombinant human QRICH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC110855

Sequence: MNNSLENTISFEEYIRVKARSVPQHRMKEFLDSLASKGPEALQEFQQTATTTMVYQQGGNCIYTDSTEVAGSLLELACPVTTSVQPQTQQEQQIQVQQPQQVQVQVQVQQSPQQVSAQLSPQLTVHQPTEQPIQVQVQIQGQAPQSAAPSIQTPSLQSPSPSQLQAAQIQVQHVQAAQQIQAAEIPEEHIPHQQIQAQLVAGQSLAGGQQIQIQTVGALSPPPSQQGSPREGERRVGTASVLQPVKKRKVDMPITVSYAISGQPVATVLAIPQGQQQSYVSLRPDLLTVDSAHLYSATGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamine-rich 1
别名 Transcriptional regulator QRICH1, Glutamine-rich protein 1, Glutamine rich protein 1, glutamine rich 1
观测分子量 85-110 kDa
GenBank蛋白编号BC110855
基因名称 QRICH1
Gene ID (NCBI) 54870
RRIDAB_3086273
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2TAL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamine-rich protein 1 (QRICH1) is a transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PMID:33384352). QRICH1 is a member of the caspase recruitment domain (CARD)-containing gene family, which encodes for proteins implicated in regulation of caspase activation in the context of apoptosis and inflammation, and in regulation of NF-κB signaling(PMID:33384352). QRICH1 can be modified by phosphorylation.

实验方案

Product Specific Protocols
IF protocol for QRICH1 antibody 30236-1-APDownload protocol
IHC protocol for QRICH1 antibody 30236-1-APDownload protocol
WB protocol for QRICH1 antibody 30236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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