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验证数据展示

  • IF Staining of human liver cancer using CL488-66399

    IF Staining of human liver cancer using CL488-66399

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL488-66399

    IF Staining of human liver cancer using CL488-66399

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-66399

    IF Staining of HepG2 using CL488-66399

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Plasminogen antibody (CL488-66399, Clone: 1F6A11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Plasminogen Monoclonal antibody

Plasminogen Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66399

产品说明书

CloneNo. 1F6A11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, IF-P

别名

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66399-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66399 targets Plasminogen in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 Plasminogen fusion protein Ag9938 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 plasminogen
别名 PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin, 1F6A11
计算分子量 810 aa, 91 kDa
观测分子量 105 kDa
GenBank蛋白编号BC060513
基因名称 Plasminogen
Gene ID (NCBI) 5340
RRIDAB_2919320
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00747
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Plasminogen antibody CL488-66399Download protocol
Standard Protocols
Click here to view our Standard Protocols