验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | λ phosphatase treated HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80706-3-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-80706-3 targets Phospho-c-SRC (Ser17) in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| 别名 | c-SRC (Ser17), p-c-SRC, p-c-SRC (Ser17), p-c-SRC(Ser17), Phospho c-SRC |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC011566 |
| 基因名称 | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P12931 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The phosphorylation of c-SRC at Ser17 is essential for its activation. This phosphorylation event is often mediated by protein kinase A (PKA) and is crucial for downstream signaling events. (PMID: 23360994)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Phospho-c-SRC (Ser17) antibody CL647-80706-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
