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验证数据展示

  • WB analysis using 86641-1-RR

    WB analysis using 86641-1-RR

    Non-treated ACHN cells and IL-4 (HZ-1004) treated ACHN cells were subjected to SDS PAGE followed by western blot with 86641-1-RR (Phospho-STAT6 (Tyr641) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • IF Staining of ACHN using 86641-1-RR

    IF Staining of ACHN using 86641-1-RR

    Immunofluorescent analysis of (4% PFA) fixed IL-4 treated ACHN cells using Phospho-STAT6 (Tyr641) antibody (86641-1-RR, Clone: 250940C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of ACHN using 86641-1-RR

    FC experiment of ACHN using 86641-1-RR

    1X10^6 ACHN cells untreated (dashed lines) or treated with IL-4 treated which intracellularly stained with 0.13 ug Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody (86641-1-RR, Clone:250940C12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody

Phospho-STAT6 (Tyr641) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 86641-1-RR

产品说明书

CloneNo. 250940C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

p STAT6 (Tyr641), Phospho STAT6, Phospho STAT6 (Tyr641), Phospho STAT6(Tyr641), Phospho-STAT6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inIL-4 treated ACHN cells
Positive IF/ICC detected inIL-4 treated ACHN cells
Positive FC (Intra) detected inIL-4 treated ACHN cells
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86641-1-RR targets Phospho-STAT6 (Tyr641) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal transducer and activator of transcription 6, interleukin-4 induced
别名 p STAT6 (Tyr641), Phospho STAT6, Phospho STAT6 (Tyr641), Phospho STAT6(Tyr641), Phospho-STAT6
计算分子量 100 kDa
观测分子量110 kDa
GenBank蛋白编号BC005823
基因名称 STAT6
Gene ID (NCBI) 6778
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42226
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. p-STAT6(Tyr641) is a biomarker of Th2-high inflammation, targeted by IL-4Rα blockers (dupilumab) and JAK1/3 inhibitors. High p-STAT6 in TAMs correlates with M2 polarization and radioresistance, blocking IL-4 or JAK1/3 decreases Tyr641 phosphorylation and restores anti-tumor immunity. (PMID: 37474750)

实验方案

Product Specific Protocols
FC protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
IF protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
WB protocol for Phospho-STAT6 (Tyr641) antibody 86641-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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