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验证数据展示

  • WB analysis using 84974-1-RR

    WB analysis using 84974-1-RR

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 84974-1-RR (Phospho-POLR2A (Ser2) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  • FC experiment of HEK-293 using 84974-1-RR

    FC experiment of HEK-293 using 84974-1-RR

    1x10^6 λ phosphatase treated HEK-293 cells were intracellularly stained with 0.06 ug Phospho-POLR2A (Ser2) Recombinant antibody (84974-1-RR, Clone:241829B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-POLR2A (Ser2) Recombinant antibody

Phospho-POLR2A (Ser2) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84974-1-RR

产品说明书

CloneNo. 241829B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

phospho-CTD, Phospho-POLR2A, Phospho-Rpb1 CTD, Phospho-Rpb1 CTD (Ser2), phospho-Ser2-CTD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, λ phosphatase treated HEK-293 cells
Positive FC (Intra) detected inλ phosphatase treated HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84974-1-RR targets Phospho-POLR2A (Ser2) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
别名 phospho-CTD, Phospho-POLR2A, Phospho-Rpb1 CTD, Phospho-Rpb1 CTD (Ser2), phospho-Ser2-CTD
计算分子量 1970 aa, 217 kDa
观测分子量250 kDa
GenBank蛋白编号BC137231
基因名称 POLR2A
Gene ID (NCBI) 5430
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24928
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)

实验方案

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser2) antibody 84974-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols