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验证数据展示

  • WB analysis using 84974-1-RR (same clone as 84974-1-PBS)

    WB analysis using 84974-1-RR (same clone as 84974-1-PBS)

    Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 84974-1-RR (Phospho-POLR2A (Ser2) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84974-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 84974-1-RR (same clone as 84974-1-PBS)

    FC experiment of HEK-293 using 84974-1-RR (same clone as 84974-1-PBS)

    1x10^6 λ phosphatase treated HEK-293 cells were intracellularly stained with 0.06 ug Phospho-POLR2A (Ser2) Recombinant antibody (84974-1-RR, Clone:241829B4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84974-1-PBS in a different storage buffer formulation.

Phospho-POLR2A (Ser2) Recombinant antibody, PBS Only

Phospho-POLR2A (Ser2) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84974-1-PBS

产品说明书

CloneNo. 241829B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84974-1-PBS targets Phospho-POLR2A (Ser2) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
别名 POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A
计算分子量 1970 aa, 217 kDa
观测分子量250 kDa
GenBank蛋白编号BC137231
基因名称 POLR2A
Gene ID (NCBI) 5430
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24928
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)