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验证数据展示

  • WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

    WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

Phospho-PKC Alpha (Thr638) Recombinant antibody, PBS Only

Phospho-PKC Alpha (Thr638) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81575-2-PBS

产品说明书

CloneNo. 241844A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

PKC alpha, PRKCA, PKCA, PKC A, EC:2.7.11.13

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

产品信息

81575-2-PBS targets Phospho-PKC Alpha (Thr638) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase C, alpha
别名 PKC alpha, PRKCA, PKCA, PKC A, EC:2.7.11.13
计算分子量 77 kDa
观测分子量77 kDa
GenBank蛋白编号AK055431
基因名称 PKC Alpha
Gene ID (NCBI) 5578
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17252
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)