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验证数据展示

  • WB analysis using 81575-2-RR

    WB analysis using 81575-2-RR

    Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-PKC Alpha (Thr638) Recombinant antibody

Phospho-PKC Alpha (Thr638) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81575-2-RR

产品说明书

CloneNo. 241844A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Phospho-PKC α Thr638, Phospho-PKC beta (Thr641), Phospho-PKC, p-PKC, p-PKC Alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, λ phosphatase treated THP-1 cells

The antibody recognizes PKC Beta-II protein phosphorylated at Thr641.

Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81575-2-RR targets Phospho-PKC Alpha (Thr638) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase C, alpha
别名 Phospho-PKC α Thr638, Phospho-PKC beta (Thr641), Phospho-PKC, p-PKC, p-PKC Alpha
计算分子量 77 kDa
观测分子量77 kDa
GenBank蛋白编号AK055431
基因名称 PKC Alpha
Gene ID (NCBI) 5578
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Alpha (Thr638) antibody 81575-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols