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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of PC-3 using 84691-1-RR (same clone as 84691-1-PBS)

    WB analysis of PC-3 using 84691-1-RR (same clone as 84691-1-PBS)

    Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 84691-1-RR (Phospho-MEK1 (Ser298)) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84691-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 84691-1-RR (same clone as 84691-1-PBS)

    FC experiment of HEK-293 using 84691-1-RR (same clone as 84691-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-MEK1 (Ser298) Recombinant antibody (84691-1-RR, Clone:241719G5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84691-1-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

Phospho-MEK1 (Ser298) Recombinant monoclonal antibody, PBS Only

Phospho-MEK1 (Ser298) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84691-1-PBS

产品说明书

CloneNo. 241719G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

产品信息

84691-1-PBS targets Phospho-MEK1 (Ser298) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 1
别名 MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2
计算分子量 43 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC139729
基因名称 MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ02750
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953)