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验证数据展示

  • Dot Blot experiment of / using 80435-8-RR

    Dot Blot experiment of / using 80435-8-RR

    Dot blot analysis was used to confirm the specificity of 80435-8-RR,Phospho-SAPK/JNK (Thr183) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis using 80435-8-RR

    WB analysis using 80435-8-RR

    Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • WB analysis of HEK-293 using 80435-8-RR

    WB analysis of HEK-293 using 80435-8-RR

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-JNK (Thr183) Recombinant monoclonal antibody

Phospho-JNK (Thr183) Uni-rAb® Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 80435-8-RR

产品说明书

CloneNo. 253546C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Dot Blot, ELISA

别名

JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inUV treated HEK-293 cells, Calyculin A treated HSC-T6 cells
Positive Dot Blot detected in/ /
Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80435-8-RR targets Phospho-JNK (Thr183) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Dot Blot, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名 JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK
计算分子量 48 kDa
观测分子量42 kDa, 50 kDa
GenBank蛋白编号NM_138982
基因名称 JNK
Gene ID (NCBI) 5599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP45983
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. Phospho-JNK (Thr183) is the activated form of c-Jun N-terminal kinase (JNK), a MAPK family serine/threonine kinase. Phosphorylation at Thr183 (often with Tyr185) by MKK4/7 activates JNK, regulating cell apoptosis, proliferation and stress responses. (PMID: 40538844)

实验方案

Product Specific Protocols
WB protocol for Phospho-JNK (Thr183) antibody 80435-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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