×
Use Able AI chat for product recommendations

验证数据展示

  • Dot Blot experiment of / using 80435-8-RR (same clone as 80435-8-PBS)

    Dot Blot experiment of / using 80435-8-RR (same clone as 80435-8-PBS)

    Dot blot analysis was used to confirm the specificity of 80435-8-RR,Phospho-SAPK/JNK (Thr183) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.

  • WB analysis using 80435-8-RR (same clone as 80435-8-PBS)

    WB analysis using 80435-8-RR (same clone as 80435-8-PBS)

    Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80435-8-RR (same clone as 80435-8-PBS)

    WB analysis of HEK-293 using 80435-8-RR (same clone as 80435-8-PBS)

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80435-8-PBS in a different storage buffer formulation.

Phospho-JNK (Thr183) Recombinant monoclonal antibody, PBS Only

Phospho-JNK (Thr183) Uni-rAb® Recombinant Antibody for WB, Dot Blot, Indirect ELISA
Cat No. 80435-8-PBS

产品说明书

CloneNo. 253546C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Dot Blot, Indirect ELISA

别名

JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

80435-8-PBS targets Phospho-JNK (Thr183) in WB, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Dot Blot, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名 JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK
计算分子量 48 kDa
观测分子量42 kDa, 50 kDa
GenBank蛋白编号NM_138982
基因名称 JNK
Gene ID (NCBI) 5599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP45983
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. Phospho-JNK (Thr183) is the activated form of c-Jun N-terminal kinase (JNK), a MAPK family serine/threonine kinase. Phosphorylation at Thr183 (often with Tyr185) by MKK4/7 activates JNK, regulating cell apoptosis, proliferation and stress responses. (PMID: 40538844)

||
New chat

Able™

正在加载，请稍候...