Phospho-IkB Alpha (Ser32/36) Recombinant antibody
Phospho-IkB Alpha (Ser32/36) Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G21
验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells, PMA treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
82349-1-RR targets Phospho-IkB Alpha (Ser32/36) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Synonyms | I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA, Phospho-IkB Alpha (Ser32/36) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004983 |
| Gene Symbol | NFKBIA |
| Gene ID (NCBI) | 4792 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha). (PMID: 16904979, PMID: 28990531 PMID: 10455908)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-IkB Alpha (Ser32/36) antibody 82349-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Oncol Endogenous hydrogen sulfide inhibition suppresses tumor growth by promoting apoptosis and pyroptosis in esophageal cancer cells | ||
Phytomedicine Ganoderma lucidum polysaccharide peptide (GLPP) attenuates rheumatic arthritis in rats through inactivating NF-κB and MAPK signaling pathways | ||
Mol Immunol Catapol attenuates the aseptic inflammatory response to hepatic I/R injury in vivo and in vitro by inhibiting the HMGB1/TLR-4/NF-κB signaling pathway via the microRNA-410-3p | ||
Am J Physiol Cell Physiol Inducible nitric oxide synthase activity mediates TNF-α-induced endothelial cell dysfunction | ||
J Ethnopharmacol The Terminalia chebula Retz extract treats hyperuricemic nephropathy by inhibiting TLR4/MyD88/NF-κB axis | ||
Int J Biol Macromol In vitro and in vivo immunomodulatory activity of acetylated polysaccharides from Cyclocarya paliurus leaves |
