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验证数据展示

  • WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis of NIH/3T3 using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • FC experiment of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    FC experiment of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-IkB Alpha (Ser32/36) Recombinant antibody (82349-1-RR, Clone:1G21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

Phospho-IkB Alpha (Ser32/36) Recombinant antibody, PBS Only

Phospho-IkB Alpha (Ser32/36) Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82349-1-PBS

产品说明书

CloneNo. 1G21

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α, p IkB α (Ser32/36)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

产品信息

82349-1-PBS targets Phospho-IkB Alpha (Ser32/36) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α, p IkB α (Ser32/36)
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004983
基因名称 IkB Alpha
Gene ID (NCBI) 4792
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25963
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).