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验证数据展示

  • WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis of NIH/3T3 using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    WB analysis using 82349-1-RR (same clone as 82349-1-PBS)

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    IHC staining of human skin cancer using 82349-1-RR (same clone as 82349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82349-1-RR (Phospho-IkB Alpha (Ser32/36) antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKBIA antibody included
  • FC experiment of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    FC experiment of HeLa using 82349-1-RR (same clone as 82349-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-IkB Alpha (Ser32/36) Recombinant antibody (82349-1-RR, Clone:1G21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82349-1-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

Phospho-IkB Alpha (Ser32/36) Recombinant monoclonal antibody, PBS Only

Phospho-IkB Alpha (Ser32/36) Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82349-1-PBS

产品说明书

CloneNo. 1G21
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α, p IkB α (Ser32/36)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NFKBIA Ready-To-Use IHC Kit. NFKBIA primary antibody included.

发表文章中的应用

WBSee 1 publications below

产品信息

82349-1-PBS targets Phospho-IkB Alpha (Ser32/36) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
别名 p IkB Alpha, p IkB Alpha (Ser32/36), p IkB Alpha Ser32/36, p IkB α, p IkB α (Ser32/36)
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004983
基因名称 IkB Alpha
Gene ID (NCBI) 4792
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25963
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. The antibody also detects the phosphorylated form of the NFKBIA (IkB Alpha) (PMID: 16904979, PMID: 28990531 PMID: 10455908).

发表文章

SpeciesApplicationTitle
mouseWB

Neoplasia

ZC3H15 suppression ameliorates bone cancer pain through inhibiting neuronal oxidative stress and microglial inflammation

Authors - Li-Quan Huang
View Article